Comparison of enzyme immunoassays and cell culture for detecting Chlamydia trachomatis.
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چکیده
منابع مشابه
Comparison of enzyme immunoassays and cell culture for detecting Chlamydia trachomatis.
Endourethral or endocervical swabs were taken from 403 patients to detect Chlamydia trachomatis by four different methods including standard tissue culture. Two immunoenzyme assays, Chlamydiazyme and IDEIA, were found to be satisfactory and could be valuable for large and busy sexually transmitted disease (STD) clinics.
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The efficiency of an immunoperoxidase serological assay and culture of Chlamydia trachomatis were compared in 127 women seeking first trimester abortion. Serum IgG and IgA antibodies specific for C trachomatis were detected by a single serovar (L2) inclusion immunoperoxidase assay (IPA). Eighty (63%) women were seropositive for chlamydial IgG and 31 (24%) for IgA antibodies. C trachomatis was i...
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Neutralization of Chlamydia trachomatis was assayed by the decrease in inclusion-forming units in baby hamster kidney cells grown in culture. Five percent fresh guinea pig sera increased neutralization titers of rabbit antisera 100- to 1,000-fold but had no effect when normal rabbit sera were tested. Neutralization of a type A or B trachoma isolate was strain specific. Neutralization by human e...
متن کاملComparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis.
A total of 201 endocervical specimens were obtained from patients with a clinical or epidemiological history suggestive of chlamydial infection. These specimens were tested by DNA probe (Gen-Probe, San Diego, Calif.) and the IDEIA III (Boots-Celltech, Berkshire, United Kingdom) monoclonal antibody enzyme immunoassay and compared with cell culture for detection of Chlamydia trachomatis. Discrepa...
متن کاملComparison of IDEIA III and cell culture for the detection of Chlamydia trachomatis in endocervical specimens.
A total of 803 endocervical samples were obtained from females with clinical or epidemiological histories suggesting chlamydia infection. These specimens were tested by IDEIA III and cell culture for the presence of Chlamydia trachomatis. After resolution of discrepant results by direct fluorescent-antibody staining of pelleted cell culture transport materials, IDEIA III demonstrated sensitivit...
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ژورنال
عنوان ژورنال: Sexually Transmitted Infections
سال: 1986
ISSN: 1368-4973
DOI: 10.1136/sti.62.3.175